A joint workshop by three labs within Taxon-omics, our RADseq workshop is organised by professors Elvira Hoerandl (U of Göttingen), Miguel Vences (TU of Braunschweig), and Natalyia Rybalka (U of Göttingen). Attendance by SPP 1991 lab members will be supported from our umbrella project funding. The goal of the workshop will be hands-on work on the analysis of RADseq data.
RAD (Restriction-site Associated DNA) Sequencing is a method for sampling the genomes of multiple individuals in a population using next generation DNA sequencing. It was invented by Eric Johnson of the University of Oregon in 2007, and has since become an important tool in collections-based systematics and evolutionary biology.
Registration deadline: 16 August 2019 (see details below)
Organizers: Elvira Hörandl (U Göttingen), Miguel Vences (U Braunschweig), Natalyia Rybalka (U Göttingen)
Accomodations: Ten rooms have been reserved at B&B Hotel Göttingen-City (hotelbb.de/de/goettingen-city, €60/night without breakfast)
Instructors: Claudia Pätzold, Kevin Karbstein, Natascha Wagner, Loïs Rancilhac
Location: PC-Pool Georg-Elias-Müller-Institut für Psychologie, Heinrich-Düker-Weg 12, 37073 Göttingen, Erdgeschoss, Raum 0.481
Program: The focus will be on downstream analyses for species delimitation, detecting hybridization, dealing with polyploidy, and using RAD data. Participants should be familiar with basics of NGS and assembly of RAD data. Block sessions will provide introductions and time for hands-on analysis of data on HPC cluster (Göttingen).
Fr 15h-18h: welcome, introduction into use of HPC cluster (Göttingen), data upload; SplitsTree (hybrids) (K. Karbstein & C. Pätzold)
Sat 9-13h: fast STRUCTURE and STRUCTURE for species, hybrids, polyploids (K. Karbstein)
Sat 14-18h: SNiPloid for polyploid and hybrid genomes (N. Wagner, C. Pätzold, K. Karbstein)
Sun 9-13h: SNAPP (and related programs if requested by participants): species delimitation using coalescence methods (Loïs Rancilhac)
Sun 14-c.16 h: free analyses according to needs of participants
We are open to further suggestions of other programs / pipelines, please communicate at the 3rd annual Taxon-omics meeting in Munich, 28-29 June 2019.
Registration: By Friday 16 August (needed for both cluster registration and final booking of accommodation). Please send to Claudia Pätzold (email@example.com).